What is the space group? Is there unmodeled density? Are all residues in your construct built? What is the refinement protocol?

FR

On Dec 18, 2009, at 6:47 AM, james09 pruza <james09x...@gmail.com> wrote:

Dear All,

I am trying to solve a 2.55 A resolution data set. The R-factor is around 24% while Free-R is 30%. The Residues in most favourable region is 95% while additionally allowed region is 5%. What are the ways to reduce the R-factor and Free-R? Around 100 water molecules are placed.

Thanks in advance for the help.

J...

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