Dear all, I have a crystal that can diffract to 3.0A for the first few frames at synchrotron, after procession only up to 3.6A data are useful. The structure has been solved by MAD. In order to get a 3.0A dataset, I am going to collect the first few images from dozens of isomorphous crystals and merge them together. Because i have no experience like this before, i am here asking for some help or suggestions. Especially I have some specific questions: 1. Is it possible to extend the resolution of the structure to 3.0A by this method? 2. At least how many frames should i collect for a single crystal for data procession? 3. Since the crystal can grow to a large dimension (0.3x0.3x0.4mm), can i collect the dataset at different positions of the crystal so that the a single crystal can be re-used for several times thus to reduce the number of total crystals needed. 4. How many frames in total should be enough for final data procession and scale. 5. which programs(hkl, xds, ...) are the best for merging the dataset. 6. is there anything i need to pay particular attention to during data collection and procession. 7. Some tricks, tips or references for such a job.
I appreciate any responses. Best Regards, lithin
