JXQI wrote: > Dear All: > > Sorry for this off-topic subject. It's not off topic. CCP4BB is for protein crystallography. Discussions of crystallographic programs are particularly ON TOPIC! But so are media, viruses, vectors and buffers...
Having said that, you might like to consider the Coot mailing list - you may get answered faster there. But, Coot questions are welcome here also. > This maybe an old topic, but I could not find the previous discussion > about it. Coot pudding? > There are some sugars, i.e.,NAD and NDG that form covalent bonds with > ASN in my protein.I tried to load the NAG by "Got Monomer" in COOT, > but I couldn't build the covalent bonds. Presumably you have merged your molecules? If you want to display the bond then you need a LINK record in your pdb file (Coot doesn't make them). If you want to use the bond in refinement, then you need "Sphere Refinement". This is discussed in the Coot User Manual. Paul.
