Hi Claudine, You could also look into the possibility that domain swapping occurred in half of your proteins. See e.g. Bennett MJ, Schlunegger MP, Eisenberg D. (1995). "3D domain swapping: a mechanism for oligomer assembly". Protein Sci 4 (12): 2455-68. PMID 8580836
Best regards, Herman -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Claudine MAYER Sent: Wednesday, January 20, 2010 12:32 PM To: [email protected] Subject: [ccp4bb] two multmeric species in solution Hi all, We have recently solved a structure that is a crystallographic dimer. We have performed UCA experiments and we observe a monomer/dimer distribution in nearly equal proportion. This distribution does not change with protein concentration, meaning that the two species are not in equilibrium. There is no structural differences between the dimer and the monomer. We would be interested in similar cases, eg coexistence of 2 multimers without exchange between the 2 forms. Does somebody have any explanation ? Thank you cheers claudine ************************************************ Pr Claudine MAYER ************************************************ Université Paris Diderot - Paris 7 Dynamique structurale des Macromolécules Département de Biologie Structurale et Chimie Institut Pasteur 25 rue du Dr Roux 75 015 PARIS Tel. 01 45 68 83 87 Mobil. 06 07 23 51 16 Fax. 01 45 68 89 93 Mail. [email protected] ************************************************
