Hi Fengxia- How about increasing the PEG% so you don't have to add as much "other" cryoprotectant?
Also, have you tried annealing the crystals? I've had success with this when I've had samples diffract similarly. The simplest way is by blocking the cryostream for 2-3 seconds (repeat 1-2 times) and then shoot. More involved way is by actually dismounting your crystals back into cryo buffer for 20-30 seconds, then remounting and shooting. HTH- Brad On Thu, Jan 21, 2010 at 1:54 AM, Fengxia Liu <fengxia....@gmail.com> wrote: > Dear all, > I am trying to diffract one semet-protein, it gave me some clear spots and > some smeared spots in one diffration, you can find maps attached. > Mother liquor condition is 0.1MTris-HCL pH8.5+10% PEG4k + 0.2M Li2SO4, I > have tried mother liquor+25%glycerol, paratone, paratone+ mineral oil, but > they all gave me such pattern. > > Does anyone know how to solve this? I appreciate any advice. > > Thank you in advance. > > Best wishes, > Fengxia > > >
