Re: [ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins

[Roger Rowlett]

You really don't need much of a system to do just affinity purification, so almost anything would be workable unless you are doing really high throughput work. On the other hand, if you need to do traditional protein purification (IEX, HIC, GEC), which would include untagged proteins, and polishing of tagged or untagged proteins, then a good chromatography system is a must. I've owned, used, and abused an AKTA FPLC since 1999. I think I have one of the first units. It is built like a tank, and is still expandable. We just upgraded the computer system and associated hardware to a modern OS (Win NT4 to XP). I don't know about the Profina units, but the AKTA system can take daily abuse with aplomb. I't been in a cold room for over 10 years and it's still going. Cheers. On 1/28/2010 11:08 AM, Sangeetha Vedula wrote: Dear all, My lab is looking into buying a protein purification system. The AKTA is more versatile, but does anyone know if the Profinia is in any way superior to the AKTA for purification of affinity-tagged proteins? If it is, I would really appreciate if you can tell me in what way it is superior. Thanks, Sangeetha. -- Roger S. Rowlett Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu