Dear list,
I'm trying to freeze crystals in cryoconditions containing the following:
0.1 M Sodium acetate pH 4.4
2.15-2.3 M Ammonium sulphate
7% n-butanol
15% glycerol
The problem is that the crystals (beautiful hexagonal prisms) seem to shatter
in a random fashion: some are unaffected, some, coming from the same drop,
disgregate miserably. One even split into three parts, of which two disgregated
and one survived perfectly well.
I've tried both by moving the crystals directly in the cryocondition and by
progressively increasing the glycerol concentration to no avail.
Shall I just select those that survive?
I was wondering if anybody has ever had this same problem and if freezing under
oil could be an alternative. If yes: any suggestions on how to fish the
crystals? I tried it in the past, but I found it very difficult...
Experiences and suggestions are welcome.
Claudia
Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia
Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039
0382 986335/8/1 Facs 0039 0382 303673
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