Dear all, Apologies for a off topic question. I could not think of a better place to ask this.
Suppose, we have four enzymes turning over five molecules, either in the living cell, or in fresh mammalian cell lyset. The molecules could be as simple as ATP, GTP, NADP, GSH etc. Now, one can add a inhibitor / drug Y to this mixture and that might change the dynamics. I am wondering how could someone measure the concentration of these metabolites.. ! It can not be done on purified enzyme, it has to be done when everything else is present. All I can think of are: a) Using mass spec on the metabolites: Quench the reaction at different time points, remove whatever possible by centrifugation and a spin filter (similar to QIAGEN kit), and do mass spec. b) Use some sort of a focused optical method (IR/UV laser?) on living cell that could excite multiple molecules (but I have no clue beyond this..) Has anybody seen something that addresses the question? Any suggestion would be highly appreciated. Cheers, Partha
