Dear All We perform fermentation using e.coli clone to obtain recombinant protein. our batch size is 20 L we perform seeeding in two phase i,.e cryovial to seed 1 then to seed 2 and then in fermentation broth. seed 2 is 10% of fermentation broth, and seed1 is 10% of seed 2. incubate each for 10 - 12 hrs. Seed media is LB media and fermentation media is complex media with vitamins
I just want to know is there a requirement for two seed, cant we just go from seed 1 to fermentation broth to reduce the passage of bacterial cell. thanks and regards
