The maltose binding protein is one possibility. Some collaborators did provide us with very pure MBP instead of the protein of interest... Mass spec eventually identified it after we wasted some time on it...
Fred > I am trying to overexpress a His-tagged protein of 29KDa in E.coli > (BL21-codon plus) and I end up with a highly expressed product that of > 43KDa that binds to the Ni-column. I also have nice crystals. Does > anyone have any experience on this. > Armando > -- Frédéric Kerff Chargé de recherche F.R.S.-FNRS Cristallographie des protéines Centre d'Ingénierie des Protéines Université de Liège - Bat B5a B4000 Liège (Sart-Tilman) Tel.: +32 (0)4 3663620 Fax: +32 (0)4 3663772
