Dear all: I am trying to create a homology model of a coiled-coil for use in molecular replacement. I have a template poly-ala coiled coil that I like to use, so that is fine. I want to thread my sequence onto the helix and am trying to find a server/easy program that will do this. I want to use a hand made alignment so that I can create 7 models shifted by residue (to be complete). Really all this should need is a template pdb and the sequence alignment. What are peoples favorite servers/easy programs for this? I have been trying to use the WHAT IF server and the SCWRL3 server, but WHAT IF isn't working for me (it just spits out the template pdb with the original sidechains that were pruned to poly ala built back in- is it because I am using a mac?) and scwrl keeps crashing with python errors (has anyone got the scwrl server to work?). Apologies for the off topic. Thanks in advance, -Tom
Tom J. Brett, PhD Assistant Professor of Medicine Division of Pulmonary and Critical Care Washington University School of Medicine Campus Box 8052, 660 S. Euclid Saint Louis, MO 63110
