I would recommend protein kinases as examples. Almost all the protein kinase activities are regulated by autophosphorylation.
In the inactive form, the kinase subdomains are aligned so that ATP cannot reach the catalytic center of the kinase. The autophosphorylation causes the two subdomains of the intrinsic kinase to shift, opening the kinase domain for ATP binding, thus activating kinase activity. Bingfa Sun ################################### PhD student, Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 P.R. China Tel: 021-5492-1117 _____ 发件人: CCP4 bulletin board [mailto:[email protected]] 代表 Paul Mcewan 发送时间: 2010年3月4日 20:21 收件人: [email protected] 主题: Re: [ccp4bb] Post-translational modification induced strcture Collagen probably isn't a great example as hydroxylation of proline in collagen enhances the thermal stability of collagen, it doesn't really "induce" collagen structure, as unhydroxylated (pro-pro-gly)n also form the canonical triple-helical structure. Unless, of course, your looking at temperatures where hydroxylated peptides form helices where unmodified ones don't. Paul.. ################################### Dr. Paul A. McEwan Office B55 Centre for Biomolecular Science University of Nottingham Nottingham NG7 2RD UK Tel: 0115 8232010 (office) Tel: 0115 8232011 (lab) <https://owa.nottingham.ac.uk/exchweb/bin/redir.asp?URL=http://www.nottingha m.ac.uk/pharmacy/research/medicinal-chemistry-struct> http://www.nottingham. ac.uk/pharmacy/research/medicinal-chemistry-struct <http://www.nottingham.ac.uk/pharmacy/research/medicinal-chemistry-structura l-biology/structbio.php> ural-biology/structbio.php ################################### _____ From: CCP4 bulletin board on behalf of Tom Murray-Rust Sent: Thu 04/03/2010 11:22 AM To: [email protected] Subject: Re: [ccp4bb] Post-translational modification induced strcture Hi Miles - I would say a good example of that phenomenon is collagen, where hydroxylation of the prolyl residues alows the polypeptide to adopt its helical conformation. In that case it is the fact there is an electronegative substituent on the prolyl ring that biases it into a particular conformation, which is perfect for forming helices (and doesn't involve a cofactor). Best Regards, Tom -- Dr. Tom Murray-Rust Department of Haematology Cambridge Institute of Medical Research Wellcome Trust/MRC Building, Hills Road Cambridge CB2 0XY On Thu, Mar 4, 2010 at 10:45 AM, Miles Pufall <[email protected]> wrote: > Dear All - > Does anyone have some good examples where a post-translational modification > has induced local structure? I'm particularly thinking of structure induced > by the modification only and not something multi-step like a modification > that recruits a cofactor that induces structure. > Thanks! > Miles > Miles Pufall > Postdoctoral Fellow, UC San Francisco > 600 16th Street, Genentech Hall S-574 > San Francisco, California 94158-2517 > > This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. __________ Information from ESET NOD32 Antivirus, version of virus signature database 4911 (20100303) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4911 (20100303) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com
