Hi Nukri, Since you have not mention red: enclosed are two pictures taken last week at GMCA before and after irradiation.
several shots of 1s with 20 micron beam, unattenuated, 12.000 keV, GM/CA at APS (23ID-B) Protein (lipidic cubic phases): 20mM HEPES pH 6.8, 200 mM NaCl, OG, diluted in monoolein Crystallization solution: 8.4 %(w/v) PEG 4000, 75 mM NaCl and 75 mM NaAc pH 5.6 ** Looks to me HEPES and some sort of salt are common. Maybe a bit lower pH then the conditions reported by Todd. David P.S: I could not allow Porto to be better then Benfica... -- David Aragão, Ph. D Membrane Structural & Functional Biology Group Unit 7, Trinity Enterprise Centre Pearse St., Dublin 2 Office phone: +35318964254 Lab phone: +35318964257 Conference & Workshop on Crystallization, Dublin, Sept. 2010, http://www.iccbm13.ie Subject: Re: Blue color upon X-ray exposure? From: "Sanishvili, Ruslan" <[log in to unmask]> To: [email protected] Reply-To: Sanishvili, Ruslan Date: Mon, 15 Mar 2010 09:23:48 -0500 Hi Todd, There are many compounds that have color centers which change color as soon as they are exposed to X-rays. Some turn, blue, some pink, some yellow etc. I'm not sure which one is the chameleon in your buffer but you can bring them separately next time and we can shoot them one by one. Cheers, Nukri Ruslan Sanishvili (Nukri), Ph.D. GM/CA-CAT Biosciences Division, ANL 9700 S. Cass Ave. Argonne, IL 60439
<<attachment: before_exposure_gmca_March2010.jpg>>
<<attachment: after_exposure_gmca_March2010.jpg>>
