Pentapeptide ligand removed from the binding site of the protein crystal and replaced with different peptide ligand Insights into Cyclin Groove Recognition: Complex Crystal Structures and Inhibitor Design through Ligand Exchange, Kontopidis, G., et. al. 2003,Structure 11 (12), pp. 1537-1546
George -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Critton, David Sent: Wednesday, March 17, 2010 6:02 PM To: [email protected] Subject: [ccp4bb] Soaking to Remove Bound Ligands from Crystals Dear ccp4bb, I recently came across a thread from 2007 discussing the removal of ions/ligands from protein crystals (https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0705&L=CCP4BB&P=R35929). The thread cited a couple of papers (Ray et al. 1991; Schreuder et al. 1988) in which crystals were grown in the presence of a given ligand, then soaked in conditions containing little (if any) ligand in order to remove the ligand from these crystals. I would like to know if there are any other reports of this method, especially if any structures were solved from pre- and post-soaked crystals, and what kinds of conformational changes (if any) accompany these kinds of soaks. Thank you in advance, David A. Critton Graduate Student, Page Laboratory Department of Molecular Biology, Cell Biology & Biochemistry Brown University Providence, RI
