I would not rely on recipes. When I pick a cryo protectant, I always prepare a set of solutions and take an image from a large loop dipped into solution. Then I pick the one with the least concentration that does not show ice rings anymore.
The behaviour can change with a crystal in the loop, but I hope by using a large loop and taking a big drop I'm on the safe side. For 2,3-butanediol I'd probably prepare the mother liquor with 10%, 15%, 20% 2,30butanediol. Having read what Eike just replied I might include the "2.5%"-steps, too. Tim On Tue, Mar 23, 2010 at 07:27:28AM +0000, David Briggs wrote: > 2,3-BD users, > > What sort of concentrations are you using? > > Dave > > -- > Delivered via an Android. > > On Mar 22, 2010 11:45 PM, "Steve Tuske" <[email protected]> wrote: > > Hi Ulrike, > > 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it > mostly seems to decrease mosaicity. I was able to switch from a > three-step soaking protocol with glycerol and glucose to a quick (30 > second to one minute) soak with 2,3-BD. > > It's a winner. > > Steve > > I > > > Hello Ulrike, > > in our lab 2,3-Butanediol has had a good reputation. > > > Tim > > On Mon, Mar 22... -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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