Hello again... I have a 2.7A resolution data set. Spacegroup P212121 as suggested by Pointless and best space group when run through Phaser. Unit cell is 110.02x160.49x186.55. Mathew's suggest 11 complexes in ASU. Both Molrep and Phaser can only find 6. This seems to be a typical observation with the complexes that we solve (high solvent content).
Due to the low(ish) resolution I wish to apply TLS and NCS during refinement. My question(s) is when to apply TLS and NCS, the order in which it should be done, and when to switch off NCS (if at all) during the stages of refinement. I have used a homologue complex (Protein A -100% identical, Protein B - 80% identical) as a model. I am open to any and all suggestions on the matter. Thanks in advance Dan
