hello everyone， recently i purify a protein conteining zinc binding
domain,and i want to determine its structure.i get the crystal,but poor
diffraction.so i try to adding zinc into the protein to optimize the
crystal,but the protein precipitate immidiately even the znic is 1
mM.BTW,we use the protein to do zinc scan,we don't find the zinc. does
anyone have some advice?hello everyone， recently i purify a protein
conteining zinc binding domain,and i want to determine its structure.i get
the crystal,but poor diffraction.so i try to adding zinc into the protein to
optimize the crystal,but the protein precipitate immidiately even the znic
is 1 mM.BTW,we use the protein to do zinc scan,we don't find the zinc. does
anyone have some advice?
