Dear all; I am working with protein which are supposed to be bound with Magnesium and Cobalt metals.I have now its crystals which are diffracting quite nicely.I want to know the Magnesium and Cobalt binding residues in the target protein. When I add magnesium or Cobalt salts to protein solution even 1mM about 50 percent of the protein precipitated out. When I soaked the crystals in about 10mM of the metals salt it lose diffraction. I varied the soaking time and the metal salt concentration,and even the types of metals salts as well but its seems that it does n,t work.
Any idea would be highly appreciated. Bashir -- Muhammad Bashir Khan Department for Biomolecular Structural Chemistry Max F. Perutz Laboratories University of Vienna Campus Vienna Biocenter 5 A-1030 Vienna Austria Phone: +43(1)427752224 Fax: +43(1)42779522
