Hello again! Following my previous question, there was something wrong with the staring model for molecular replacement. Now that is sorted, I have 8 complexes in the ASU. After a few rounds of refinement with NCS and isotropic Bfactors, both the Rfactor and Rfree get stuck at 30% and 36%, respectively.
I have only just noticed that I am trying to model ~43000 atoms with ~95000 unique reflections (98% complete, at 2.58 Angstrom resolution). Is this the reason why the R factors are stuck and I should start the refinement again from scratch using overall Bfactors with NCS and switch to TLS once my Rfactor is less than 30% and possibly reduce the number of reflections used for Rfree (currently using the standard CCP4 5%) Any input would be greatly appreciated! Dan
