On Tue, Apr 13, 2010 at 09:24:13AM -0400, Nathaniel Clark wrote: > [...] If you don't want to buy those tools just try 2 nylon loops, and lots > of patience. Or use cats whiskers which you can fix into a Pasteur pipette with wax, nail varnish, plasticine, etc.
I find them to have just the right stiffness to work with crystals, the tip is very pointy and does not fray. You just need to know a cat owner and ask them to look out for the whiskers the next time they vaccuum clean their flat. Tim > Nat > > On Tue, Apr 13, 2010 at 4:49 AM, Enrico Stura <[email protected]> wrote: > > Amit Sharma, > > > > I second Tim's suggestions. > > > > The first experiment is the following. > > Set up several experiments at constantly decreasing precipitant > > concentrations. > > Streak seed all of them. If in any of those you can get non-clustered > > crystals > > non matter what size, seeding can solve your problem. > > To restore crystal size follow the rest of Tim's list. > > > > Enrico. > > > > > > On Mon, 12 Apr 2010 23:01:10 +0200, Tim Gruene <[email protected]> > > wrote: > > > >> Have you tried anything, yet? The list of answers can be quite long. > >> A couple of keywords: > >> - micro-seeding > >> - macro-seeding > >> - cross-seeding > >> - additive screens > >> - extend to purification protocol > >> - change temperature, pH, etc. > >> - have you searched around the current conditions > >> ... > >> > >> if you describe a little what you have done so far we can also get an idea > >> of > >> your status of experience and adapt the answers accordingly. > >> > >> Kind regards, Tim > > > > -- > > Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office > > Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab > > LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE > > http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura > > http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html > > e-mail: [email protected] Fax: 33 (0)1 69 08 90 71 > > -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A
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