Dear Jurgen and Ho Leung To add few more point regarding my question:
1. Crystal was first frozen in LN2 and then transfered to cryo stream (in presence of LN2 in vial) 2. Anealing did not help (both short time and long time) - perhaps the crystal dies. 3. Spots are clear to available resolution (is: 6-7A). In the high resolution region there is no spot but looks like smear in the whole area. 4. The crystal was approximately 1.0mm length and 0.4mm dia. I mounted on 0.5mm loop. So the liquid around the crystal was very less. I deliberately avoided more solvent in the loop to help diffraction. Thanks Syed --- On Thu, 4/15/10, Jürgen Bosch <[email protected]> wrote: From: Jürgen Bosch <[email protected]> Subject: Re: [ccp4bb] Cryo Vs crystal size To: [email protected] Date: Thursday, April 15, 2010, 3:46 AM There are a couple of additional factors not taken into account here. 1. LN2 versus frozen in strem or propane etc2. did you try to flash anneal the larger crystal3. smeary diffraction from the big crystal or not ?4. how much residual solvent was around your crystal when freezing ? In general smaller crystals are anyhow better in my hands. Jürgen On Apr 14, 2010, at 5:36 PM, syed ibrahim wrote: Hi All I had two crystals grown in same well, one is small and other is 10 times bigger. I treated both crystal in same cryo and same time. The smaller one diffracted to 2.5A and the bigger one to 6-7A. I was expecting the bigger one to diffract high resolution. I assume the bigger crystal might have lot of solvent which prevent for high resolution. If it is true what could be the best way to dehydrate crystal without affecting crystal quality? Thank you Syed PS: Taken care of less solvent to be present in the loop -Jürgen BoschJohns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
