Can you soak the crystal in a solution lacking 0.2M lithium sulfate (since it isn't your precipitant)-perhaps add 0.2M sodium chloride to maintain ionic strength- then in a high concentration of your ligand solution? Nat
On Fri, Apr 23, 2010 at 3:26 PM, Jim Pflugrath <[email protected]> wrote: > I would test several hypotheses. You have a multitude of salts to choose > from. Test them all. > Let me ask you this: What anions can you test? What's already on your > shelf of chemicals? What did you test already? > > Jim > ________________________________ > From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of > Yi-Liang Liu > Sent: Friday, April 23, 2010 12:53 PM > To: [email protected] > Subject: [ccp4bb] Substitute for Lithium Sulfate > > Hi Everyone, > > I have a question about the crystallization condition. Currently the > condition I use contains 0.2M lithium sulfate. However, the ligand there > seems to compete the site where sulfate binds in the active site. Are there > any good substitute to replace the Lithium sulfate in my crystallization > buffer? > Best, > Lucas > >
