Sorry for the mistake. It is co-crystallised with protein. And there is difference in the cell parameter between, apo protein and the co-crystallised structure. The difference is 10A in one axis and 5A at another axis, third axis are same in both crystal.
Thank you Sajid ________________________________ From: Christina Bourne <[email protected]> To: sajid akthar <[email protected]> Sent: Thu, 29 April, 2010 4:53:50 PM Subject: Re: [ccp4bb] NADP behaviour in crystal Maybe the soak wasn't successful? NADP usually sits in a groove that may not be accessible in your crystal form; perhaps it's just sticking around the outside of the protein in quasi-ordered fashion... ________________________________ From: sajid akthar <[email protected]> To: [email protected] Sent: Thu, April 29, 2010 3:06:26 PM Subject: [ccp4bb] NADP behaviour in crystal Hi All I had a soaked crystal with NADP; and the crystal diffracted to 2.5A. I was looking for NADP at its binding site, I came up with wierd density which I could not fit with any end of NADP. The density is upto 3.5 A in Fo-Fc map; and is spherical long enough to put nicotine. But the density is not flat. I found some density at the surface (which is not the typical binding site for NADP), where I can fit the two phophate groups as well as two sugar rings next to each phophate. But I do not see any density either Nicotine part or the nucleotide part. The crystal grown in pH 8.0. I guess NADP may undergo hydrolysis at pH 8.0 resulting in ADP and nicotine; I failed to fit these products as well. Has anyone experienced like this? and your suggestions are welcome Thank you Sajid
