Dear all,
I am working on protein-DNA complex of which I finally got crystals,
which diffract to at about 2 A. Unfortunately I wasn't able (so far)
to solve the structure by MR, since the sequence identity to known
homologues is very low (below 25%).
By now I have successfully produced SeMet crystals and am in the
process to optimize the data collection strategy, however I would also
like to work with brominated DNA.
Since I have never worked with a protein-DNA complex beforehand my
expertise is still limited.
Therefore I was wondering if people could help me on that topic:
Is there any summarizing paper about structure solution using Br-DNA?
What are the rules in general I have to follow, e.g. which nucleotide
is the best one too choose for bromination and why?
The DNA sequence (30mer in total) and the region that binds to the
protein is known, but I have no information about any structural
implication on the DNA molecule (e.g. kinks introduced whilst binding).
Any help/ suggestion would be greatly appreciated
sincerely,
Mareike
Dr Mareike Kurz
[email protected]
Department of Biochemistry
University of Zürich, Irchel
Winterthurstrasse 190
8057 Zürich, Switzerland
+41446356557
