Dear Arpita, For Getting rid of DNA you can incubate the lysate with DNase and incubate it before lysing the cells with sonication or french press (or anyother method of your choice)
cheers Rashmi On Wed, May 12, 2010 at 8:53 PM, Arpit Mishra <[email protected]> wrote: > > > ---------- Forwarded message ---------- > From: Arpit Mishra <[email protected]> > Date: Wed, May 12, 2010 at 6:20 PM > Subject: regarding purification of DNA binding protein > To: [email protected] > > > hi all > i am trying to purify DNA binding protein, but i couldnt get rid of the DNA > after performing hi-trap heparin column, i have also tried high conc salt > buffer (upto 1 M NaCl) and also tried o.2 M lithium sulphate, please suggest > me some way to get rid of undesired DNA.. > > thanks > > Regards > > Arpit > > > -- rashmi
