Dear friends, here I'm summarizing all of your suggestions regarding dissolving a hydrophobic ligand (in this case, containig benzoic acid moities) for crystallization and biochemical assays:
1)Sodium salts of benzoic acid containing molecules can often be soluble in water to high concentration.If material is not limiting, it is a strong possibility to be explored. 2)In case of a ligand with a protonated group (like benzoic acid), increasing the pH of the medium helps the group to donate proton, in turn forming carboxylate anaion, which dissolves better.The need to elevate pH will vary depending upon the ligand. 3)Using low mol. wt. PEGs, such as 200, 300, 350 etc. to dissolve the ligand. PEG mono methyl ether 350, 550 can also be tried. 4) using mild detergents (DDM, OG etc.) 5)(For xtalization, not for activity....) Keep prot concentration as low as 1 mg/ml, then add you ligand in low concentration according to molar equivalence as req. After incubation (for whatever time required), concentrate the solution, then use for xtalization.Final conc. of DMSO shouldn't exceed 2 - 5%. Thanks to all for your time, I will try out and let know. Regards, Tuhin. -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean.
