On May 18, 2010, at 4:13 PM, Jay Pan wrote:
Hello Everyone,
I have a reasonably well fitted electron density map through
molecular replacement. However, there is always some red region left
no matter how hard I tried when the mtz file is loaded into Coot. Is
this because my model is still not good enough or it’s natural to
most model fittings.
Negative density (with absolute value above 2 sigma) sitting on atoms
is much more of a concern than random negative peaks floating in the
solvent breeze, as it indicates misplacement of the model.
In another word, should I be worried about the red region? Thanks in
advance.
I've alway found it very helpful to worry about absolutely everything
obsessively to the point where it causes endoderm to bleed. Which is
why, I suppose, Paul chose red.
Jay
