Hi Andre, We saw this type of crystals quite often during crystallization of our 300 kDa complex. To our experience these crystals diffract very poorly (~7-10 A) and anisotropically. They may have highly favorable crystal contacts in one direction than the other two directions and grow very fast in favorable direction. To our experience we could not improve the diffraction significantly for these crystals. However, it is possible that in case of small protein, the diffraction can be improved.
Best luck, ravi Ravindra D. Makde present address: Scientific Officer E High Pressure & Synchrotron Radiation Physics Division Bhabha Atomic Research Centre, Trombay, Mumbai, India. Tel: +91-22-25506754 (Res.), "All that we are is the result of what we have thought. The mind is everything. What we think, we become" Buddha --- On Wed, 6/2/10, Andre Ambrosio <[email protected]> wrote: From: Andre Ambrosio <[email protected]> Subject: [ccp4bb] Off-topic: hollow protein crystals To: [email protected] Date: Wednesday, June 2, 2010, 3:07 PM Dear all, We have recently obtained crystals from a small protein, and interestingly, at least for me, they are hollow trigonal rods (please see pictures attached). Just out of curiosity, has anybody ever seen such feature for protein crystals before? Regards, -Andre.
