Thanks to all those who kindly answered my original message, both to the board and private.
It seems that hollow crystals are rather common and as most people pointed out, may be due to fast growth. Indeed, ours grew overnight from a initial screening at room temperature and with very high protein concentration, so we are in the process of optimization at the moment. We also have tested the "drinking straws" in the picture in one of our beamlines and got diffraction to a fairly decent resolution and I am hopeful we will have the protein structure very soon. Kind regards, -Andre. De: CCP4 bulletin board [mailto:[email protected]] Em nome de Andre Ambrosio Enviada em: quarta-feira, 2 de junho de 2010 16:07 Para: [email protected] Assunto: [ccp4bb] Off-topic: hollow protein crystals Dear all, We have recently obtained crystals from a small protein, and interestingly, at least for me, they are hollow trigonal rods (please see pictures attached). Just out of curiosity, has anybody ever seen such feature for protein crystals before? Regards, -Andre.
