The H+-ATPase from A. thaliana had anisotropic diffraction (ca.
3.6/5.5Å). This could be explained by the crystal packing and more
specifically the detergent micelles in the packing.
See:
Pedersen BP, Buch-Pedersen MJ, Morth JP, Palmgren MG, Nissen P. Crystal
structure of the plasma membrane proton pump. Nature 450, pp. 1111-4,
(2007).
Pedersen BP, Morth JP, Nissen P. Structure determination using poorly
diffracting membrane protein crystals - Lessons from the H+ and
Na+,K+-ATPases. Acta Cryst D, 66, pp. 309-313, (2010).
-Bjørn
On 2010-06-08 16:38, Matthias Zebisch wrote:
Dear everybody!
I am currently having an issue with anisotropic diffraction and would
like to cite some references.
I am sure there are plenty of examples outthere.
So, if you are having an own published example at hand, can you please
send me the reference and
maybe the diffraction limits along good and bad directions as well?
Thank you a lot,
Matthias
