On 9 Jun 2010, at 12:54, Murray, James W wrote: > I was wondering if anyone has compiled a list of common proteins that can > co-purify from E. coli preps (and yeast etc), and crystallize instead of the > protein of interest. If list members send me individual examples I will > compile a summary for the bulletin board.
Biochim Biophys Acta. 2006 Sep;1760(9):1304-13. Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography. Bolanos-Garcia VM, Davies OR. (the first hit on google for "common proteins that can co-purify from E. coli and crystallize") is a good start. There's also: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt 10):880-5. There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization. Veesler D, Blangy S, Cambillau C, Sciara G. Huw -- Dr Huw Jenkins Astbury Centre for Structural Molecular Biology University of Leeds
