Hi Everyone, I have some problem in refining a structure. The data goes to 2.4A (with some 30% completeness at 2.15A), the structure was solved by MR with Phaser, refinement was done with Phenix, but the r and r-free are now staying at 26% and 32%, even with all possible waters and missing fragments added. Data was collected at APS at cryo condition. One thing I noticed during HKL2000 data processing was that the chi^2 were way too high at lower resolutions shells, I had to adjust the default error model in HKL2000 to get the chi^2 to around 1, but this adjustment reduced the overall I/sigI ratio a lot (from around 20 to 5).
The quality of electron density maps looks fine to me for a 2.4 A data set and I was able to build all the missing CDR loops for the antibody in the complex. I am lost now, should I just re-collect a new data set? Thanks, Tongqing Tongqing Zhou, Ph.D. Staff Scientist Structural Biology Section Vaccine Research Center, NIAID/NIH Building 40, Room 4607B 40 Convent Drive, MSC3027 Bethesda, MD 20892 (301) 594-8710 (Tel) (301) 793-0794 (Cell) (301) 480-2658 (Fax) ****************************************************************** The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives. ******************************************************************
