Actually it probably would be better ask the supplier of your screen
first how they got PEG 1K diluted to 12.5%. If they did hit it then you
have to do the same. But even better idea is to order that PEG 1K from
the same company that sells the screens. It will insure identical
treatment.
My 2 cents.
Vaheh
________________________________
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of
R.Srinivasan
Sent: Wednesday, June 23, 2010 3:11 AM
To: [email protected]
Subject: [ccp4bb] PEG 1000
Dear All,
I have got initial crystals in a condition with PEG 1000. The
PEG 1000 stock we had in our lab was rock solid and when i heated it to
about 50 degrees for 15 to 20 minutes it became a solution. We thought
the compound has got out dated or something like that and bought a brand
new bottle from Sigma and this is rock solid too.
Is this something characteristic of PEG1000? The hit condition
says its 12.5% w/v PEG 1000 but it apparently seems i could never get a
powder of it.
So my question is, Can i go ahead using this melted solution form
of PEG1000 for setting up optimizations?
Thank you all in anticiaption,
Vasan
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