Yes, I know. I am already working on some other modifications, or analogues. The questions was only to know some experience from different labs. Thanks.
Petr 2010/7/5 Vellieux Frederic <[email protected]> > Petr Kolenko wrote: > >> Dear all, >> >> I am working on crystallization of a protein purified using intein-chitin >> binding domain. There is 86% identity with protein with already known >> high-resolution structure, but I am not able to get any crystals. I heard >> that reversed phase chromatography is not good for crystallization, e.g. Has >> anyone of you observed something similar for intein-chitin chromatography? >> Is there any serious "structural" damage to the target protein during the >> purification? >> >> -- >> >> >> Petr Kolenko >> [email protected] <mailto:[email protected]> >> http://kolda.webz.cz >> > The failure in crystallisation can be due to so many reasons... including > protein damage (ending up with a mixture of components is probably one of > the worse cases) Sometimes you need to modify your protein in order to > crystallise it, or even use an analog from another organism. > > Fred. > -- Petr Kolenko [email protected] http://kolda.webz.cz
