Fellow CCPers, Slightly off-topic question: I recently produced several large batches of B834 auxotrophic E. coli cells in minimal media to make selenomethionine-labeled protein. While growing, the cells had a slightly pink color to them. After cell lysis and centrifugation at 12,000xg, a bright red band of pigment is present in the pellet. Has anyone experienced this red pigmentation before while using this strain (or perhaps know what the pigment might be)? When grown in LB or TB media the same cells show no pigmentation.
-- Jim Fairman, Ph D. Post-Doctoral Fellow National Institutes of Health - NIDDK Cell: 1-865-748-8672 Lab: 1-301-594-9229 E-mail: [email protected] [email protected]
