Have you picked from multiple images and do you have more than 1000 reflections to index from? You might also want to limit the resolution manually to perhaps 3 first. Before processing your data you should refine your cell by using multiple wedges of 10 degrees. Check out the manual for additional keywords.
Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Jul 20, 2010, at 10:03 PM, Sampath Natarajan wrote: > > Dear All, > > I have the problem in processing the data with imosflm. The data was > collected with 2.5A resolution about 300 images. Auto index found the space > group P222 with unit cell a= 40.2; b= 79.2; c= 194.7 and α=b=γ = 90.0. For > this cell, the penalty is 7. I feel this penalty is good enough to select the > cell based from the list. When I start to refine the cell, the new popup > window is opening with some suggestions as below. > > “The index process has failed. It might be worthwhile trying again with” > > 1. A larger or smaller largest cell edge > 2. Using more or fewer reflections (200-1000 is best) > 3. Using more and / or different images > 4. Checking your direct beam position carefully > > Could anyone help me to solve these problems? > > Thanks, > > Sincerely > > Sampath N >
