*** these jobs are not for my group, I am posting this on request from
my colleague Prof. Peter Peters, an electron microscopist. Do not
reply to me. If you reply to me I promise I will not forward your
email to Peter ... consider it as form of pre-selection of
candidates ... ***
Peter Peters email: [email protected]
The Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital
(NKI-AVL) is for the group of Prof. Peter J. Peters in the department
of Cell Biology, looking for a Postdoc.
The cellular nanocosm is made up of numerous types of macromolecular
complexes or biological nanomachines. These form functional modules
that are organized into complex subcellular networks. Information on
the ultra-structure of these nanomachines has mainly been obtained by
analyzing isolated structures, using imaging techniques such as X-ray
crystallography, NMR, or single particle electron microscopy (EM). Yet
there is a strong need to image biological complexes in a native state
and within a cellular environment, in order to gain a better
understanding of their functions. Emerging methods in EM are now
making this goal reachable. Cryo-electron tomography is approaching
macromolecular resolution. X-ray data can be 'docked' into the lower
resolution particle density maps to create a macromolecular atlas of
the cell under normal and pathological conditions. The majority of
cells, however, are too thick to be imaged in an intact state and
therefore methods such as 'high pressure freezing' with 'cryo-
sectioning of unperturbed vitreous fully hydrated samples' have been
introduced for electron tomography. The candidate will join a new EU
project, which has the goal to image biological nano-machines and
their mode of action within stem cells at macromolecular-scale. The
identification and analysis of pathways of toxicological relevance in
organotypic cell cultures is the ultimate aim of this consortium.
Challenges include visualizing individual conformations (imposed by
drugs) of GFP tagged macromolecular complexes and using volumetric
classification algorithms based on maximum likelihood statistical
approach. Our initial attempt at understanding the spatial
arrangements of the ribosome in situ is exciting and hints at the
possibilities of imaging the vast majority of other biological
machines. With new technologies being developed by us and others a
resolution of 2.5 nm is approaching. We recently obtained grants for 2
Krios Titan cryo-TEMs thus creating a great infrastructure. For more
details see www.necen.nl
The Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital
(NKI-AVL) is for the group of Prof. Peter J. Peters in the department
of Cell Biology, looking for a Postdoc for an on-going project.
The candidate will join an on-going project, which has the ultimate
goal to image biological nano-machines and their mode of action at
macromolecular-scale by pushing the current limits of visual
proteomics and nanotechnologies. Electron Microscopy, bioinformatics,
template matching and image processing are part of the new ventures.
As cryo electron tomography is approaching macromolecular resolution,
X-ray data can be 'docked' or fitted into the lower resolution density
maps to create a macromolecular atlas of the cell under normal and
pathological conditions. The majority of cells, however, are too thick
to be imaged in an intact state and therefore we have built nano-
fluidic chambers of 200 nm thickness in which mammalian cells
voluntarily migrate before they are vitrified. We now aim to develop
and use methods for visualizing vitreous biological samples for cryo-
LM and cryo-TEM and examine the sub-cellular distribution and
organization of macromolecular structures involved in cell migration
and the organization of cytoskeletal polymers. This is a project in
collaboration with Dr. Gerard van Veen, FEI, and Prof. Marileen
Dogterom, AMOLF, and the science faculty of the University of Delft
and Leiden.
The Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital
(NKI-AVL) is for the group of Prof. Peter J. Peters in the department
of Cell Biology, looking for a PhD candidate - Amsterdam, the
Netherlands
To develop and use methods for visualizing vitreous biological samples
for cryo-LM and cryo-TEM and examine the sub-cellular distribution and
organization of macromolecular structures involved in phagocytosis of
mycobacteria or CTL / target cell synaptic interactions, depending on
personal interest.
The candidate will join an on-going project, which has the ultimate
goal to image biological nano-machines and their mode of action within
cells at macromolecular-scale by pushing the current limits of visual
proteomics and nanotechnologies. Electron Microscopy, bioinformatics,
template matching and image processing are part of the new ventures.
As cryo electron tomography is approaching macromolecular resolution,
X-ray and NMR data can be 'docked' or fitted into the lower resolution
density maps to create a macromolecular atlas of the cell under normal
and pathological conditions. The majority of cells, however, are too
thick to be imaged in an intact state and therefore we have built nano-
fluidic chambers of 200 nm thickness in which mammalian cells
voluntarily migrate before they are vitrified. The project has yield a
very successful first part, where the technology developed so far
proved to be suitable for light-microscopy observations. This is a
project in collaboration with Dr. G van Veen, FEI, and Prof B. Koster,
University of Leiden.
Peter J. Peters
Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital |
Plesmanlaan 121 - B6 | 1066CX Amsterdam
Mobile: +31 6 5538 4705 | www.nki.nl/research/peters | www.necen.nl |
www.postdoc-development.eu
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