Dear Hari,
You might look at Lucy Waskell's experiences with full length
mammalian Cytochrome P450 2B4. While she got >50 mg of purified
protein per liter of culture, many other heme proteins are much harder
to express in E. coli with heme assembly proteins, chaperones, etc.
You just have to try it. However, for cytochrome P450s, you might
want to resort to some molecular engineering to increase expression (a
la Eric Johnson and co.; look of cytochrome P450 2C9 structure for
example).
Saribas, Gruenke, and Waskell (2001) Overexpression and Purification
of the Membrane-Bound Cytochrome P450 2B4. Protein Expression and
Purification 21, 303–309.
Good luck,
Michael
****************************************************************
R. Michael Garavito, Ph.D.
Professor of Biochemistry & Molecular Biology
513 Biochemistry Bldg.
Michigan State University
East Lansing, MI 48824-1319
Office: (517) 355-9724 Lab: (517) 353-9125
FAX: (517) 353-9334 Email: rmgarav...@gmail.com
****************************************************************
On Aug 26, 2010, at 12:56 PM, Hari Namboodiri wrote:
Hi CCPers
Can anyone provide insights about expressing heme containing
proteins in E.col? Does E.coli need any porphyrin precursor during
expression or you need special E.coli strains. I have references
mentioning delta-aminolevulinic acid for one ortholog but none for
another. The enzyme is CYP51.
Thanks
Hari
