1. Does the packing of crystal lattice allow binding of inhibitor? There are several structures of this enzyme and inhibitors published, our crystal crystallized in different conditions but the SG are similiar, let's say, P4(3) or P4(2), P2 or P1.
2. Is the inhibitor sensitive to radiation? Some are stable under radiation. 3. Is the assignment of space group correct? We do have problem about SP. The protein is a dimer, the structure can be refined pretty well (R/Rfree close to 0.23) with two different SP (P4(2) dimer and P42(1)2(1) monmer). I checked with POINTLESS and it seems P42121 is a better option. But both didn't contain any extra density. 4. Do you have inhibitor in cryo/mounting solution? No, I just sock the crystal very quickly (1S) in 30% glycerol. 5. Did you try co-crystallization? Yes and No, all are empty. ----- 原始邮件 ----- 发件人: "Tongqing Zhou (NIH/VRC) [E]" <[email protected]> 收件人: "[email protected]" <[email protected]> 发送时间: 星期六, 2010年 8 月 28日 下午 5:29:13 主题: Your Ccp4 question Xiaopeng, Somehow I could not reply to the original message. 1. Does the packing of crystal lattice allow binding of inhibitor? 2. Is the inhibitor sensitive to radiation? 3. Is the assignment of space group correct? 4. Do you have inhibitor in cryo/mounting solution? 5. Did you try co-crystallization? Good luck. Tongqing Tongqing Zhou, Ph.D. Staff Scientist Structural Biology Section Vaccine Research Center, NIAID/NIH Building 40, Room 4607B 40 Convent Drive, MSC 3027 Bethesda, MD 20892 (301) 594-8710 (Tel) (301) 793-0794 (Cell) (301) 480-2658 (Fax) -- -- 胡小鹏 理学博士 教授 中山大学药学院结构生物学实验室 广东省 广州市 番禺区 大学城 510006 Tel: 020-39943032 Xiaopeng Hu, D.Sc., Professor School of Pharmaceutical Sciences Sun Yat-sen University, Higher Education Mega Center Guangzhou, Guangdong, China 510006 Tel. +86 020 39943032
