Hi Qing,
just want to add that it also may depend on the redundancy of the data.
While good MAD experiments were performed with low redundancy (best to
use a strategy for data collection) a successful SAD experiment often
depends on high redundancy.
Best Regards,
Georg
Am 30.08.2010 20:58, schrieb Phoebe Rice:
Dear Qing,
Many structures have been solved that way. Make sure you try solvent
modification (flattening / flipping) on your map. This is because SAD will
give two equally-probably estimates for the phase. The initial, unmodified map
will use the average of the two, but solve modification should be able to break
the amibiguity.
It sounds like you're new to crystallography - do you have a local advisor
to get help from?
Phoebe
=====================================
Phoebe A. Rice
Dept. of Biochemistry& Molecular Biology
The University of Chicago
phone 773 834 1723
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
http://www.rsc.org/shop/books/2008/9780854042722.asp
---- Original message ----
Date: Mon, 30 Aug 2010 20:32:26 +0200
From: CCP4 bulletin board<[email protected]> (on behalf of Jane
Bailey<[email protected]>)
Subject: [ccp4bb] About SAD phasing
To: [email protected]
Hi, All
I would like to ask whether is it possible to
allocate the Se site and obtain the phase by just
using the SAD data set (no native dataset used)?
Thanks,
Qing
