Dear,
Because this can certainly be of interest to other crystallographers,
I would like to post the following positive comment:
The recently developed software ARCIMBOLDO: Crystallographic Ab
Initio protein solution far below atomic resolution,
(Rodriguez et al., Nature Methods, 6, 651, 2009)
http://chango.ibmb.csic.es/ARCIMBOLDO/
was able to find a successful solution for one of our protein
structures.
The protein showed only 30% sequence identity and ,although we've
built several homology models, failed all MR attempts.
Furthermore, crystal data were complete (1.6 angstrom), showed high
redundancy (cubic space group) and lot's of alpha helices.
In the future, a public web server will be provided to run the
software on, but in the meantime very kind help was provided by the
authors Dayté Rodriguez and Isabel Uson.
I hope this comment will stimulate the use and the further
development/implementation of the program.
Regards
Kristof
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Kristof Van Hecke, PhD
Biomoleculaire Architectuur
Celestijnenlaan 200 F
B-3001 Heverlee (Leuven)
Tel: +32(0)16327477
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