I use PISA to analyse this - sometimes the differences depend on the
definition of "what is a hydrogen bond?" and unless you have very high
resolution it is risky to say there are significant differences.. But
certainly there are examples where the results are very significant
indeed - you could look at the extensive heamoglobin literature..
Eleanor
Roger Rowlett wrote:
I don't think this is uncommon at all. For example, we published a structure
where 10 chains did not bind ligand at all, and 2 chains did in the ASU (see PDB
3E3I). We have also recently solved a structure where two active sites in the
ASU are in different states.
Cheers.
On 9/8/2010 12:50 PM, Rongjin Guan wrote:
Dear All,
I have a structure with two complexes in the asymmetric unit, and the interactions
on the interface are not the same in the two complexes. Briefly, there are two
additional
hydrogen bonds in one complex, but not in the other. This coule be due to
crystallization
artefact, but may have other explanations.
Can anyone direct us to some references where this has been discussed before?
Thank you very much
Rongjin Guan
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Roger S. Rowlett
Professor
Department of Chemistry
Colgate University
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Hamilton, NY 13346
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