Dear Xingliang,
This is vaguely reminiscent to a pattern that was observed by Zora
Markovic-Housley on precession picture of crystals of ornithine
aminotransferase: the even layer (l=2n) had a nice pattern diffracting
to high resolution while the odd layer (l=2n+1) had a honeycomb stucture
with the hole at the centre positioned were a diffraction spot was expected.
Reference:
Marković-Housley Z, Kania M, Lustig A, Vincent MG, Jansonius JN, John RA.
Eur J Biochem. 1987 Jan 15;162(2):345-50.
Quaternary structure of ornithine aminotransferase in solution and
preliminary crystallographic data.
Daniel
Le 14/09/2010 02:31, xingliang zhang a écrit :
Dear everyone,
Recently，we collected data of a native protein crystal on synchrotron
in Shanghai. When we did with the original data with HKL2000, we found
an unconversant phenomenon ,just as the picture in the enclosure, there
were some grid indicated by arrows appearing on the low resolution
areas.The crytal grew in 20%PEG3000，100mMTris-Cl，200mM Ca（OAc）2
，and the cryo-protectant is 20% sucrose mixed with well buffer。Who can
tell me the reasons it appear the grid ？because of the sucrose? I’ll
appreciated for any explains and suggestion.
Best wishes
xingliangzhang
--
Best wishes!
xingliangcheung,PhD,candidate
Protein crystallography Lab ,College of biological sciences ,China
agricultural university.
No. 2 yuanmingyuan west road HaidianDistrict, Beijing, 100193
Tel:01062814122,
E-mail:[email protected] <mailto:e-mail%[email protected]>
