dear Maria, we routinely use Mosquito for MMS, the ratio works the best: 100nl protein + multi-aspirate mode x (15nl seeds + 85nl reservoir solution). best regards, alexey Alexey RAK
Structural Biology SDI/LGCR Sanofi-aventis R&D Centre de Recherche Paris 13, Quai Jules Guesde - BP 14 94403 Vitry sur Seine Cedex France Phone : +33 (0)1 58 93 86 93 Fax : +33 (0)1 58 93 80 63 Mobile : +33 (0)6 26 71 28 60 Email : alexey....@sanofi-aventis.com ________________________________ From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Steiner, Roberto Sent: Monday, September 20, 2010 10:21 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Micromatrix seeding using the mosquito Dear Maria In our hands Mosquito MMS worked by adding 2x protein solution + 1x seed solution + 1x reservoir. The reason for doing this was two-fold: I) ratio protein/reservoir is similar to the condition that generated the seed stock II) a deliberate bias toward the initial condition is given I) and II) might not always be helpful and possibly other ratios should be explored. Best wishes Roberto On 20 Sep 2010, at 08:55, "Maria Håkansson" <maria.hakans...@maxlab.lu.se> wrote: Dear all, Anyone who have had success using the mosquito for micromatrix seeding? Which way is the optimal way of adding seed solution to sitting drops on an MRC plate? Is it better to add 100 nl protein + 10 nl seed solution + 100 nl reservoir solution or to add concentrated seed solution (1 microliter) directly to the reservoir solution and then pipett 100 nl protein + 100 nl reservoir solution. Best regards and thanks in advance, Maria Håkansson __________________________________________ Maria Håkansson, Ph.D. Senior Scientist, Max-lab, Lund University Phone: +46 (0) 76 8585 706 Fax: +46 (0) 46 222 47 10 Ole Römers väg 1 (P.O. Box 188) SE-221 00 Lund, Sweden Web address: <http://www.maxlab.lu.se> www.maxlab.lu.se Email: <mailto:maria.hakans...@maxlab.lu.se> maria.hakans...@maxlab.lu.se __________________________________________
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