Dear All,
Thank you for all your replies, I think there are some additional
informations about this screening to help judgment:
1. this screening was set in a 0.2+0.2 microliter drop, so the
microcrystals(?) are very small.
2. these things came out in different conditions, after 1-4 days, and
didnot grow bigger.
3. the protein buffer contains 500mM NaCl while most of the screen
conditions has ethanol, so could them be NaCl crystals?
One more question: has anybody experienced that the N-terminal histag
cannot be cut off, but it is really there and
helps the purification? Is any information can be achieved from this?
Thanks a lot!
Best
Yang
On Mon, Sep 20, 2010 at 6:28 PM, yang li <[email protected]> wrote:
> Dear All,
>
> After crystal screening I got something look like microcrystals but
> not sure, picture taken by the robot
> is attached. I am new in protein crystallization, I wonder if anybody can
> give me a guidance about this
> situation, and how to optimize the condition if they are real protein
> crystals.
> Any suggestion will be appreciated!
>
> Best
> Yang
>
