Hi Tri, To me, the first 3 pictures suggest that there is a faint greenish tinge in the little shapes (hopefully crystalline) there. I guess the idea of the Topaz chip is that you can take it as is to the x-ray beam or am I wrong?
Cheers, Boaz ----- Original Message ----- From: Tri Ngo <[email protected]> Date: Wednesday, September 29, 2010 10:50 Subject: [ccp4bb] The color of GFP crystals? To: [email protected] > Dear CCP4 community, > > I wonder if anybody has experience about the color of GFP > crystals. Is there > any chance that the GFP crystal will become colorless? We are > working on one > membrane protein fused with GFP. Since we got some crystals > which didn't > show any color (Condition 01), I am not sure if I should go for > optimization. > > Please check the link below for the image details: > http://www.docstoc.com/docs/55950590/GFP-crystals > > Because of the limited amount of sample, we used Topaz system > (screeningchip) to do the screening and there is no way to > confirm these crystals are > actually protein crystals. I noticed that the crystals are > bigger day by > day. Is it a good sign to confirm the protein crystals? > > Thank you very much for your helpful information! > > Best wishes, > > * > TriNgo > > * > > Structural Biology Lab > > School of Medicine - Sungkyunkwan University > > Phone: 031-299-6150 > Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel Phone: 972-8-647-2220 ; Fax: 646-1710 Skype: boaz.shaanan
