Dear Mario, what is the resolution of the data? Could you try SeMet-MAD/SAD or some other phasing method to overcome the MR-problem?
Tim On Thu, Sep 30, 2010 at 12:54:36PM +0100, Mario Milani wrote: > Dear all, > i have a 30 kDa protein that crystallize so far in three different conditions > but with the same space group. It initially looks like tetragonal (I4, a=141, > b=141, c=208) and then results triclinic (P1, a=141, b=141 c=144, alpha=119, > beta=119, gamma=90), hosting about 24 mol. in the unit cell. Other data: self > rotation shows the presence of 4 peaks with chi=180; molecular replacement > shows the presence of a pseudo-translation peak; DLS made at protein > concentration close to crystal growth conditions shows a Rh compatible with > something like a tetramer with low polydispersity (about 15%). Do you have > any experience with similar ‘asymmetric’ associations? Do you have any > suggestions, beside the addition of ligands to the crystal growth conditions, > in order to get a ‘simpler’ crystallographic assembly? I have some models > (with sequence identity less than 25%) in order to try MR but all trials so > far did not solve the structure (using balbes, molrep, phaser and epmr). Any > suggestion is welcome. > Thank you, > > Mario Milani -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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