Hi Flip, Following your suggestion, I send the link to download the screenshots of the ligand density: http://twitpic.com/2tn35i
Best regards Priscila ________________________________________ From: Flip Hoedemaeker [fhoe...@googlemail.com] On Behalf Of Flip Hoedemaeker [f...@formulatrix.com] Sent: Friday, October 01, 2010 9:18 AM To: Priscila Oliveira de Giuseppe Subject: Re: [ccp4bb] unknown density Hi Priscilla, I don't think a lot of people will take the trouble to find out for themselves which format your map files have... Its better to send screen shots, better still to put your screenshots on a social media page (facebook, twitpic, whatever) and email the link. Flip On 10/1/2010 13:12, Priscila Oliveira de Giuseppe wrote: > Hi, everyone, > > I solved a structure at 1.7 A. After fitting all residues into the model, I > found an extra density at the catalytic site. The components of the > crystallization condition did not explain it (crystallization condition: 40% > PEG200, 0.1 M Tris-HCl, protein buffer: PBS) > > Neither substrates nor products of this enzyme fitted this density. > > I modelled two PEG molecules with half occupancy. They fitted very well, but > I am not convinced that this is the best explanation. > > Does anyone figure out what this density can be? Attached to this e-mail I > send the maps and a pdb file with the residues surrounding the ligand density. > > > > Thanks > > Priscila
