Hello everyone
first I want to thank you guys in advance. I am trying to co-crystallize a 12
KDa protein with a 2 KDa peptide. The biochemistry studies showed the binding
should be 1:1 . but the binding is not very strong ( Kd is around 20-40 uM). I
am wondering, is there any bad effect of excessive peptide in the solution?
someone suggested I use lot of peptide , say 3 times of the peptide to protein.
usually what is the ratio you will try when you deal with this not-so-small
ligand ?
any suggestion is appreciated.
Lei Feng
