Dear CCP4ers, After I make Molecular replacement with Phaser and combine the resulting mtz and the anomalous .mtz using CAD, I obtain an anomalous map which shows me good anomalous signal. I'm displaying this map in coot by choosing in the expert mode the labels DANO and PHWT (or PHIC together with FOM). So far so good, the problem comes when I start my refinement using Phenix. After the refinement round I combine again my anomalous .mtz and the mtz file from the phenix round. Then I go to coot and choose the labels DANO and PH2FOFCWT, but then what I see is a map that looks more like noise.
Can somebody tell me if I'm doing something wrong?. Should I only use the anomalous map after the phaser round ? I appreciate all your comments. thanks Maiclo,
